The pilot dataset contains 43 samples; 11 healthy volunteers, 32 IPAH cases. These were analysed using read lengths of 75 and 150bp in independent sequencing runs. Both these datasets were analysed to identify differentially expressed genes.

Differential expression

DEG comparison

DEG Plots

## Warning: Ignoring 19683 observations
## Warning: Ignoring 21728 observations

Reactome pathway enrichment

The differentially expressed transcripts were mapped to EntrezIDs and then tested for enrichment in Reactome pathways.